Microcosmic Study of Nickel Stress towards Soil Bacteria and their Biochemical Characterization

Zeeshan Ahmad, Abid Ur Rehman, Muhammad Anees

Abstract


Heavy metal contamination of soil decreases microbial diversity and causes bacterial communities to lose part of their degradative capabilities. The present work is mainly related to Nickel (Ni) toxicity. Ni is the twenty fourth most abundant element of the earth’s crust and has been detected in the biosphere. High concentration of Nickel frequently inhibits enzymatic activity, DNA replication, transcription and translation by binding to proteins and nucleic acids. We investigated the impact of Ni-stress on the soil bacterial populations in artificial microcosmic conditions as well as compared the artificially created stress with the natural long term heavy metal stress to bacterial populations. Microcosmic conditions were provided by using sterile test tubes for two different intervals (24 and 48 hours) by addition of 20g soil and Ni treatments (500, 1000 and 1500 ppm). Our results showed that Ni stress significantly reduce the bacterial densities as compared to control without Ni contamination. The impact of Ni increased with increase in its concentration. The isolates were picked and characterized using different biochemical tests. The bacterial strains isolated in the study may be useful for bioremediation of heavy metals. Similarly, identifying the enzymes produced in the Ni resistant strains will also be an important future perspective.

 


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ISSN: 2311-4630

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